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Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at 2 μg/mL.
Immunohistochemical staining of human brain tissue using BAP3 antibody at 2.5 μg/mL.
Immunofluorescence of BAP3 in human brain tissue with BAP3 antibody at 20 μg/mL.
Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at 2 μg/mL.
Trial Size

BAP3 Antibody

CATALOG NUMBER: 4503

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
BAP3 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human BAP3.
The immunogen is located within the first 50 amino acids of BAP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BAP3 antibody can be used for the detection of BAP3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
BAP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BAP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BAP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BAIAP3
Additional Names:
BAP3 Antibody: BAP3, KIAA0734, BAI1-associated protein 3, BAP3
Protein Accession Number:
BAA34710
Protein Gi Number:
3929917
Ncbi Gene Id Number:
8938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAP3 Antibody: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Background Reference 1:
Shiratsuchi T, Oda K, Nishimori H, et al. Cloning and characterization of BAP (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Comm.1998; 251:158-65.
Background Reference 2:
Palmer RE, Lee SB, Wong JC, et al. Induction of BAIAP3 by the EWS-WT1 chimeric fusion implicates regulated exocytosis in tumorigenesis. Cancer Cell2002; 2:497-505.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4503

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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