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<strong>Figure 1 Western Blot Validation in Rat Lung Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: BAP31, 3675 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: BAP31, 3675 (0.5 μg/mL), BAP31, 64-237 (2 μg/mL), and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: BAP31, 3675 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of BAP31 in Rat Lung Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-BAP31 antibody (3675) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation in Rat Lung Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: BAP31, 3675 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

BAP31 Antibody

CATALOG NUMBER: 3675

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-BAP31 antibody (3675) was raised against a peptide corresponding to 16 amino acids near the center of human BAP31.
The immunogen is located within amino acids 130 - 180 of BAP31.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 0.5-2 μg/mL; IHC: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 3:
Cat. No. 1223 - Caco-2 Cell Lysate
Positive Control 4:
Cat. No. 1282 - 3T3/NIH Cell Lysate
Predicted Molecular Weight:
Predicted: 28 or 34kD
Observed: 28 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BAP31 expression profile in human cell lines detected by two independent anti-BAP31 antibodies that recognize different epitopes, 3675 against central domain and 64-237, against recombinant protein.  BAP31 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies.

Isoforms:
Human BAP31 has 2 isoforms, including isoform 1 (246aa, 28kD) and isoform 2 (313aa, 35kD). Mouse BAP31 has one isoform (245aa, 28kD) and Rat BAP31 also has one isoform (245aa, 28kD). 3675 can detect human, mouse and rat.
Purification:
BAP31 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BAP31 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCAP31
Additional Names:
BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31
Protein Accession Number:
NP_005736
Protein Gi Number:
32171186
Ncbi Gene Id Number:
10134
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.
Background Reference 1:
Ng et al. J. Cell Biol.1997; 139:327-38.
Background Reference 2:
Kim et al. EMBO J.1994; 13:3793-800.
Background Reference 3:
Zen et al. J. Biol. Chem.2004; 279:44924-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3675

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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