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Formalin paraffin human skin stained with Basic Cytokeratin antibody (KRTH/1076).
Formalin paraffin rat stomach stained with Basic Cytokeratin antibody (KRTH/1076).
Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).
SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone KRTH/1076). Confirmation of Integrity and Purity of the Antibody.
Formalin paraffin human skin stained with Basic Cytokeratin antibody (KRTH/1076).

Basic Cytokeratin Antibody [KRTH/1076]

CATALOG NUMBER: 33-549

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Rat
Immunogen:
Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Application Note:
ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
KRT76
Additional Names:
KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, CK-2P, HUMCYT2A
Ncbi Gene Id Number:
51350
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-549

List Size:
100 ug

List Price:

$626.00

Datasheet
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