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42-051 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-051 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

BAT3 Antibody

CATALOG NUMBER: 42-051

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-PETNAPNHPSPAEY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments in Human Brain (Frontal Cortex) and Human Ovary lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Spleen shows nucleoli staining in splenocytes. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_004630.3; NP_542433.1). Reported variants representing identical protein: NP_542434.1, NP_001092004.1, NP_542433.1 (isoform b).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAT3
Additional Names:
BAT3, HLA-B associated transcript 3, BAG-6, BAG6, D6S52E, G3, HLA-B associated transcript-3, large proline-rich protein BAT3, scythe
Protein Accession Number:
NP_004630.3,NP_542433.1
Protein Gi Number:
149158692
Ncbi Gene Id Number:
7917
Background Reference 1:
Annunziata CM, Kleinberg L, Davidson B, Berner A, Gius D, Tchabo N, Steinberg SM, Kohn EC. BAG-4/SODD and associated antiapoptotic proteins are linked to aggressiveness of epithelial ovarian cancer. Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6585-92.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-051

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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