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Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.
Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.
Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.
Trial Size

BATF Antibody

CATALOG NUMBER: 7069

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF.
The immunogen is located within the first 50 amino acids of BATF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
14 kDa
Purification:
BATF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BATF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BATF
Additional Names:
BATF Antibody: SFA2, B-ATF, BATF1, SFA-2, Basic leucine zipper transcriptional factor ATF-like, B-cell-activating transcription factor
Protein Accession Number:
NP_006390
Protein Gi Number:
5453563
Ncbi Gene Id Number:
10538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BATF Antibody: BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.
Background Reference 1:
Dorsey MJ, Tae HJ, Sollenberger KG, et al. B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family. Oncogene 1995; 11:2255-65.
Background Reference 2:
Echlin DR, Tae HJ, Mitin N, et al. B-ATF functions as a negative regulator of AP-1 mediated transcription and blocks cellular transformation by Ras and Fos. Oncogene 2000; 19:1752-63.
Background Reference 3:
Schraml BU, Hildner K, Ise W, et al. The AP-1 transcription factor Batf controls TH17 differentiation. Nature 2009; 460:405-9.
Background Reference 4:
Ise W, Kohyama M, Schraml BU, et al. The transcription factor BATF controls the global regulators of class-switch recombination in both B cells and T cells. Nat. Immunol. 2011; 12:536-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7069

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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