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Total cell lysates from HL60 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bax-UNLB (Cat. No. 99-622). Proteins were visualized using Goat Anti-Mouse IgG1, Human ads-HRP secondary antibody and chemiluminescent detection.
Total cell lysates from HL60 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bax-UNLB (Cat. No. 99-622). Proteins were visualized using Goat Anti-Mouse IgG1, Human ads-HRP secondary antibody and chemiluminescent detection.

Bax Antibody [2D2]

CATALOG NUMBER: 99-622

Clonality:
Monoclonal
Tested Applications:
Flow
Host Species:
Mouse
Species Reactivity:
Bovine, Dog, Human, Monkey
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Dog, Human, Monkey
Immunogen:
Human Bax peptide (a.a. 3-16)
Conjugate:
Unconjugated
Tested Applications:
Flow
Application Note:
Bax Antibody [2D2] for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, purification, and neutralization assays.
Specificity:
Bax
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.1 mg/mL
Storage Conditions:
Store vial at 2-8°C
Ncbi Official Symbol:
BAX
Additional Names:
Apoptosis regulator BAX, BCL2 associated X protein
Protein Accession Number:
Q07812
Ncbi Gene Id Number:
581
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 2D2 reacts with an N-terminal epitope between amino acids 3-16 of human Bax. It can bind both soluble and detergent-altered forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

99-622

List Size:
0.1 mg

List Price:

$369.00

Out of stock

Datasheet
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