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Antibody used in IHC on Human kidney.
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)).
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in IHC on Human, Mouse, Rat.
Antibody used in IHC on Human kidney.

BAX Antibody

CATALOG NUMBER: 31-014

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BAX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
BAX antibody can be used for detection of BAX by ELISA at 1:62500. BAX antibody can be used for detection of BAX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
21 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store BAX antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
BAX
Additional Names:
BAX, BCL2L4
Protein Accession Number:
NP_620116
Protein Gi Number:
20631958
Ncbi Gene Id Number:
581
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Background Reference 1:
Yamaguchi, H., et al., (2004) J. Biol. Chem. 279 (38), 39431-39437.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-014

List Size:
100 ul

List Price:

$473.00

Datasheet
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