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IHC testing of FFPE human melanoma with Bax antibody (clone ARBX-1)
IHC testing of FFPE Hodgkin's Lymphoma with Bax antibody (clone ARBX-1)
IHC testing of FFPE human melanoma with Bax antibody (clone ARBX-1)

Bax Antibody [ARBX-1]

CATALOG NUMBER: 33-964

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant full-length human protein was used as the immunogen for the Bax antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
Immunofluorescence: 1-2 ug/ml
Western blot: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Bax antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificity:
Does not react with mouse and rat
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
BAX
Additional Names:
Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
Ncbi Gene Id Number:
581
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
BCL2 associated X protein/Bax accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-964

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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