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45-322 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-322 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

BAZ2B Antibody

CATALOG NUMBER: 45-322

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-YFEKKWTDTFKVS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 35kDa band in Testis and Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 221kDa according to NP_038478. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Small Intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2ug/ml.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAZ2B
Additional Names:
BAZ2B, bromodomain adjacent to zinc finger domain, 2B, DKFZP434H071, DKFZp762I0516, FLJ45644, WALp4
Protein Accession Number:
NP_038478.2
Protein Gi Number:
94681063
Ncbi Gene Id Number:
29994
Background Reference 1:
Jones MH, Hamana N, Nezu J, Shimane M. A novel family of bromodomain genes. Genomics. 2000 Jan 1;63(1):40-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-322

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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