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Western blot analysis of lysate from K562 cell line, using BBS5 Antibody at 1:1000.
Western blot analysis of lysate from K562 cell line, using BBS5 Antibody at 1:1000.

BBS5 Antibody

CATALOG NUMBER: 61-020

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse
Immunogen:
This BBS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids from the Central region of human BBS5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BBS5
Additional Names:
Bardet-Biedl syndrome 5 protein, BBS5
Protein Accession Number:
Q8N3I7
Protein Gi Number:
74750959
Ncbi Gene Id Number:
129880
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.
Background Reference 1:
Li J.B.,et al.Cell 117:541-552(2004).
Background Reference 2:
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Background Reference 3:
Hillier L.W.,et al.Nature 434:724-731(2005).
Background Reference 4:
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-020

List Size:
400 ul

List Price:

$495.00

Datasheet
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