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43-375 (1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-375 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-375 (1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BCAR1 Antibody

CATALOG NUMBER: 43-375

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KQTSTFSKQTPHH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCAR1
Additional Names:
BCAR1, breast cancer anti-estrogen resistance 1, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas scaffolding protein family member 1, Crk-associated substrate p130Cas, breast cancer anti-estrogen resistance protein 1
Protein Accession Number:
NP_001164185.1,NP_001164186.1,NP_001164187.1,NP_001164188.1,NP_001164189.1,NP_055382.2,NP_001164190.1,NP_001164191.1,NP_001164192.1
Protein Gi Number:
44662836
Ncbi Gene Id Number:
9564
Background Reference 1:
Huang W, Deng B, Wang RW, Tan QY, He Y, Jiang YG, Zhou JH.BCAR1 protein plays important roles in carcinogenesis and predicts poor prognosis in non-small-cell lung cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-375

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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