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45-324 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-324 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BCAR3 Antibody

CATALOG NUMBER: 45-324

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RKLEPPPVKQAEL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Mouse embryonic Kidney epithelial cell lysates (calculated MW of 92.6kDa according to Human NP_003558.1) and 92.3kDa according to Mouse NP_038895.1. Recommended concentration: 0.5-2ug/ml. Data kindly provided by anonymous customer.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCAR3
Additional Names:
BCAR3, NSP2, SH2D3B, breast cancer anti-estrogen resistance 3, KIAA0554, OTTHUMP00000011961, breast cancer antiestrogen resistance 3, dJ1033H22.2 (breast cancer anti-estrogen resistance 3)
Protein Accession Number:
NP_003558.1
Protein Gi Number:
4502371
Ncbi Gene Id Number:
8412
Background Reference 1:
van Agthoven T, van Agthoven TL, Dekker A, van der Spek PJ, Vreede L, Dorssers LC. Identification of BCAR3 by a random search for genes involved in antiestrogen resistance of human breast cancer cells. EMBO J. 1998 May 15;17(10):2799-808.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-324

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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