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Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BCAR3 Antibody

CATALOG NUMBER: 6999

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3.
The immunogen is located within amino acids 30 - 80 of BCAR3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
91 kDa
Purification:
BCAR3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BCAR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BCAR3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCAR3
Additional Names:
BCAR3 Antibody: NSP2, SH2D3B, NSP2, UNQ271/PRO308, Breast cancer anti-estrogen resistance protein 3, Novel SH2-containing protein 2
Protein Accession Number:
NP_003558
Protein Gi Number:
4502371
Ncbi Gene Id Number:
8412
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.
Background Reference 1:
van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.
Background Reference 2:
Riggins RB, Quilliam LA, and Bouton AH. Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3. J. Biol. Chem. 2003; 278:28264-73.
Background Reference 3:
Schuh NR, Guerrero MS, Schrecengost RS, et al. BCAR3 regulates Src/p130Cas association, Src kinase activity, and breast cancer adhesion signaling. J. Biol. Chem. 2010; 285:2309-17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6999

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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