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Western blot analysis of extracts of various cell lines, using BCAS3 antibody (22-827) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using BCAS3 antibody (22-827). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAS3 antibody (22-827). Western blot was performed from the immunoprecipitate using BCAS3 antibody (22-827) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using BCAS3 antibody (22-827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

BCAS3 Antibody

CATALOG NUMBER: 22-827

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
BT474
Positive Control 3:
Rat brain
Predicted Molecular Weight:
Observed: 115kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BCAS3
Additional Names:
BCAS3 Antibody: MAAB, GAOB1, Breast carcinoma-amplified sequence 3
Ncbi Gene Id Number:
54828
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-827

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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