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Western Blot at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
BCAT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.

BCAT1 Antibody

CATALOG NUMBER: 55-059

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This BCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-107 amino acids from the Central region of human BCAT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCAT1
Additional Names:
Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), Protein ECA39, BCAT1, BCT1, ECA39
Protein Accession Number:
P54687
Protein Gi Number:
215274162
Ncbi Gene Id Number:
586
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the cytosolic form of the enzymebranched-chain amino acid transaminase. This enzyme catalyzes thereversible transamination of branched-chain alpha-keto acids tobranched-chain L-amino acids essential for cell growth. Twodifferent clinical disorders have been attributed to a defect ofbranched-chain amino acid transamination: hypervalinemia andhyperleucine-isoleucinemia. As there is also a gene encoding amitochondrial form of this enzyme, mutations in either gene maycontribute to these disorders. Alternatively spliced transcriptvariants have been described.
Background Reference 1:
Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Barber, M.J., et al. PLoS ONE(3), E9763 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-059

List Size:
400 ul

List Price:

$495.00

Datasheet
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