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Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.

BCDIN3D Antibody

CATALOG NUMBER: 61-351

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000-2000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCDIN3D
Additional Names:
Pre-miRNA 5'-monophosphate methyltransferase, 2.1.1.-, BCDIN3 domain-containing protein, BCDIN3D
Protein Accession Number:
Q7Z5W3
Protein Gi Number:
74738762
Ncbi Gene Id Number:
144233
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.
Background Reference 1:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 2:
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Background Reference 3:
Xhemalce B.,et al.Cell 151:278-288(2012).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-351

List Size:
200 ul

List Price:

$495.00

Datasheet
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