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Western blot analysis in Hela cell line lysates (35ug/lane).
BCKDHB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of BCKDHB using rabbit polyclonal BCKDHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of BCKDHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in Hela cell line lysates (35ug/lane).

BCKDHB Antibody

CATALOG NUMBER: 55-327

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCKDHB
Additional Names:
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain, BCKDE1B, BCKDH E1-beta, BCKDHB
Protein Accession Number:
P21953
Protein Gi Number:
129034
Ncbi Gene Id Number:
594
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Branched-chain keto acid dehydrogenase is a multienzymecomplex associated with the inner membrane of mitochondria, andfunctions in the catabolism of branched-chain amino acids. Thecomplex consists of multiple copies of 3 components: branched-chainalpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2)and lipoamide dehydrogenase (E3). This gene encodes the E1 betasubunit, and mutations therein have been associated with maplesyrup urine disease (MSUD), type 1B, a disease characterized by amaple syrup odor to the urine in addition to mental and physicalretardation, and feeding problems.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Gorzelany, K., et al. Turk. J. Pediatr. 51(2):97-102(2009)
Background Reference 3:
Quental, S., et al. Mol. Genet. Metab. 94(2):148-156(2008)
Background Reference 4:
Kang, H., et al. Fertil. Steril. 89(3):728-731(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-327

List Size:
400 ul

List Price:

$495.00

Datasheet
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