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Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Antibody used in WB on Human Brain at 0.2-1 ug/ml.

BCKDK Antibody

CATALOG NUMBER: 30-795

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCKDK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
BCKDK antibody can be used for detection of BCKDK by ELISA at 1:62500. BCKDK antibody can be used for detection of BCKDK by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store BCKDK antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
BCKDK
Additional Names:
BCKDK, BDK, BCKDKD
Protein Accession Number:
NP_005872
Protein Gi Number:
171906589
Ncbi Gene Id Number:
10295
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.
Background Reference 1:
Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-795

List Size:
100 ul

List Price:

$519.00

Datasheet
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