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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Bcl-2 3337, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Bcl-2 3335, (2 μg/mL), Bcl-2 3337, (2 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Bcl-2 3337, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunocytochemistry Validation of Bcl-2 in Mouse A-20 Cells</strong><br>Immunocytochemical analysis of Mouse A-20 cells using anti-Bcl-2 antibody (3337) at 2 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: Bcl-2 3337, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells
Green Product
IAV
Trial Size

Bcl-2 Antibody

CATALOG NUMBER: 3337

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (93%), Chicken: (86%)
Immunogen:
Anti-Bcl-2 antibody (3337) was raised against a peptide corresponding to 14 amino acids near the center of human Bcl-2.
The immunogen is located within amino acids 80-130 of Bcl-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
WB: 1-4μg/mL; ICC: 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3:
Cat. No. 1208 - THP-1 Cell Lysate
Predicted Molecular Weight:
Predicted: 26kD
Observed: 26 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain.  Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). 3337 can detect all isoforms of human, mouse and rat
Purification:
Bcl-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Bcl-2 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bcl-2 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCL2
Additional Names:
Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
Protein Accession Number:
AAH27258
Protein Gi Number:
20072668
Ncbi Gene Id Number:
596
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
Background Reference 1:
Lockshin et al. Cell Death Differ. 2000; 7:2-7.
Background Reference 2:
Cory et al. Oncogene 2003; 22:8590-607.
Background Reference 3:
Heiser et al. Exp. Geron. 2004; 39:1125-35.
Background Reference 4:
Hockenbery et al. Nature 1990; 348:334-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3337

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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