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Western blot analysis of extracts from HeLa cells treated with Nocodazole using Phospho-BCL-2 (Ser87) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa cells treated with Nocodazole using Phospho-BCL-2 (Ser87) Antibody. The lane on the right is treated with the antigen-specific peptide.

BCL-2 (phospho Ser87) Antibody

CATALOG NUMBER: 79-992

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BCL-2 (phospho Ser87) antibody was raised against a peptide sequence around phosphorylation site of serine 87 (A-L-S (p)-P-V) derived from Human BCL-2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of BCL-2 only when phosphorylated at serine 87.
Predicted Molecular Weight:
28 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BCL2
Additional Names:
Apoptosis regulator Bcl-2, BCL2
Protein Accession Number:
NP_000624.2
Protein Gi Number:
72198189
Ncbi Gene Id Number:
596
Background:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Background Reference 1:
Nencioni L, et al. (2009)J Biol Chem 284, 16004-15. Pattingre S, et al. (2009)J Biol Chem 284, 2719-28. Wei Y, et al. (2008)Mol Cell 30, 678-88.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-992

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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