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Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL.
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Bcl-B Antibody

CATALOG NUMBER: 3163

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B.
The immunogen is located within amino acids 90 - 140 of Bcl-B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 26 kDa
Purification:
Bcl-B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bcl-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCL2L10
Additional Names:
Bcl-B Antibody: Boo, Diva, BCL-B, BCLB, Bcl-2-like protein 10, Anti-apoptotic protein NrH, Bcl2-L-10
Protein Accession Number:
NP_065129
Protein Gi Number:
9966783
Ncbi Gene Id Number:
10017
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
Background Reference 1:
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
Background Reference 2:
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Background Reference 3:
Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
Background Reference 4:
Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3163

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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