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Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.
Trial Size

Bcl-G Antibody

CATALOG NUMBER: 3165

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G.
The immunogen is located within the first 50 amino acids of Bcl-G.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Positive Control 1:
Cat. No. 1215 - U937 Cell Lysate
Positive Control 2:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Purification:
Bcl-G Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bcl-G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCL2L14
Additional Names:
Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14
Protein Accession Number:
NM_030766
Protein Gi Number:
13540528
Ncbi Gene Id Number:
79370
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
Background Reference 1:
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Background Reference 2:
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
Background Reference 3:
Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3165

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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