Bcl-rambo Antibody [Rocky-2]

CATALOG NUMBER: XA-1031

Clonality:
Monoclonal
Tested Applications:
ICC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Bcl-rambo (monoclonal Rocky-2) antibody was raised against recombinant human Bcl-rambo.
Conjugate:
Unconjugated
Tested Applications:
ICC, IP, WB
Application Note:
Bcl-rambo antibody can be used to recognize the C-terminus of human Bcl-rambo (aa. 224-450). It detects endogenous protein by Immunocytochemistry (1:300), Immunoprecipitation (1:100) and Western Blot (1:1,000). Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection. This product is for research use only.
Purification:
Bcl-rambo (monoclonal Rocky-2) antibody was purified from concentrated hybridoma tissue culture supernatant. Bcl-rambo (monoclonal Rocky-1) antibody was purified to ≥95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
Bcl-rambo antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
23786
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem. 276, 19548 (2001).
Background Reference 2:
Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death: P. Yi, et al.; FEBS Lett. 534, 61 (2003).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1031

List Size:
0.1 mg

List Price:

$515.00

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