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Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (Cat. No. 99-620). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP secondary antibody and chemiluminescent detection.
Total cell lysates from mouse thymocytes and Hep G2 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bcl-xL-UNLB (Cat. No. 99-620). Proteins were visualized using Goat Anti-Mouse IgG2a, Human ads-HRP secondary antibody and chemiluminescent detection.

Bcl-xL Antibody [2H12]

CATALOG NUMBER: 99-620

Clonality:
Monoclonal
Tested Applications:
Activity, Flow, ICC, IHC-Fr, IHC-P, IP
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Bcl-xL peptide (a.a. 3-14) common to mouse and human
Conjugate:
Unconjugated
Tested Applications:
Activity, Flow, ICC, IHC-Fr, IHC-P, IP
Application Note:
Bcl-xL Antibody [2H12] for use in flow cytometry, immunohistochemistry, ELISA, western blotting, and immunoprecipitation assays.
Specificity:
Bcl-xL
Clonality:
Monoclonal
Isotype:
IgG2a
physical-state:
Liquid
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.1 mg/mL
Storage Conditions:
Store vial at 2-8°C
Ncbi Official Symbol:
BCL2L1
Additional Names:
Apoptosis regulator Bcl-X, Bcl-2-like 1 protein, BCL2L1
Protein Accession Number:
Q07817
Ncbi Gene Id Number:
598
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bcl-xL is a 29 kDa member of the Bcl-2 family of proteins that has been shown to block apoptosis. The monoclonal antibody 2H12 reacts with an N-terminal epitope between amino acids 3-14 that is common to mouse, human, and rat Bcl-xL.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

99-620

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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