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45-020 (0.1ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-020 (0.1ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BCL2 Antibody

CATALOG NUMBER: 45-020

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: AGRTGYDNREIVMKYC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 27+25kDa band observed in Human Lymph lysates (calculated MW of 26.3kDa according to NP_000624.2 and of 22.2kDa according to NP_000648.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_000624; NP_000648)
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCL2
Additional Names:
BCL2, Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, OTTHUMP00000163680
Protein Accession Number:
NP_000624.2,NP_000648.2
Protein Gi Number:
72198189
Ncbi Gene Id Number:
596
Background Reference 1:
Reed JC. Double identity for proteins of the Bcl-2 family. Nature. 1997 Jun 19;387(6635):773-6. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-020

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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