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Western blot analysis of extracts of mouse intestine, using BCL2L2 antibody (15-008) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using BCL2L2 antibody (15-008) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using BCL2L2 antibody (15-008) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BCL2L2 antibody (15-008) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse intestine, using BCL2L2 antibody (15-008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

BCL2L2 Antibody

CATALOG NUMBER: 15-008

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human BCL2L2 (NP_001186768.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse intestine
Predicted Molecular Weight:
Observed: 27kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BCL2L2
Additional Names:
Bcl-2-like protein 2, Bcl2-L-2, Apoptosis regulator Bcl-W, BCL2L2, BCLW, KIAA0271
Ncbi Gene Id Number:
599
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-008

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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