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45-325 staining (1ug/ml) of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-325 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit
45-325 Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
45-325 staining (1ug/ml) of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BCL7A Antibody

CATALOG NUMBER: 45-325

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-MKLEASQQNSEEM
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Lymph Node lysates (calculated MW of 25.0kDa according to NP_066273.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCL7A
Additional Names:
BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7
Protein Accession Number:
NP_066273.1,NP_001019979.1
Protein Gi Number:
10337613
Ncbi Gene Id Number:
605
Background Reference 1:
Zani VJ, Asou N, Jadayel D, Heward JM, Shipley J, Nacheva E, Takasuki K, Catovsky D, Dyer MJ. Molecular cloning of complex chromosomal translocation t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon. Blood 1996 Apr 15;87(8):3124-34

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-325

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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