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<strong> Figure 1 WB Validation in Human Tissues</strong><br>Loading: 10 μg of lysate Antibodies: BCMA, 2397, 2 μ g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Immunohistochemistry Validation of BCMA in Human Colon</strong><br> Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BCMA antibody (2397) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 3 Immunofluorescence Validation of BCMA in Human Spleen</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling BCMA with 2397 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 4 Immunohistochemistry Validation of BCMA in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BCMA antibody (2397) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in Human Tissues
Loading: 10 μg of lysate Antibodies: BCMA, 2397, 2 μ g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

BCMA Antibody

CATALOG NUMBER: 2397

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Anti-BCMA antibody (2397) was raised against a peptide corresponding to 16 amino acid synthetic peptide near the carboxy terminus of human BCMA.

The immunogen is located within the last 50 amino acids of BCMA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P: 1-5 μg/mL; IHC-P/IF: 10-20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 20 kD

Observed: 22 kD
Purification:
BCMA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BCMA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BCMA antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNFRSF17
Additional Names:
BCMA Antibody: BCM, BCMA, CD269, TNFRSF13A, BCM, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein
Protein Accession Number:
NP_001183
Protein Gi Number:
23238192
Ncbi Gene Id Number:
608
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BCMA Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non-immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFF-R, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-κB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.
Background Reference 1:
Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999; 285:260-3
Background Reference 2:
Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J. Exp. Med. 1999; 189:1747-56
Background Reference 3:
Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J. Leukoc. Biol. 1999; 65:680-3
Background Reference 4:
Mukhopadhyay A, Ni J, Zhai Y, et al. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-κB, and c-Jun NH2-terminal kinase. J. Biol. Chem. 1999; 274:15978-81

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2397

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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