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45-326 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
45-326 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and weak cytoplasmic staining. The nuclear
45-326 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)

BCOR Antibody

CATALOG NUMBER: 45-326

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: LSATPLYGNVHSW-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Strong expression of the protein seen in the nucleus of U2OS and A431 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms. Variants (NP_060215.4; NP_001116855.1) encode the same isoform.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCOR
Additional Names:
BCOR, BCL-6 interacting corepressor, FLJ20285, KIAA1575, BCL6 co-repressor, ANOP2, FLJ38041, MAA2, MGC131961, MGC71031, 5830466J11Rik, 8430401K06Rik, MCOPS2
Protein Accession Number:
NP_060215.4,NP_001116855.1,NP_001116856.1,NP_001116857.1
Protein Gi Number:
21071037
Ncbi Gene Id Number:
54880
Background Reference 1:
Tang TT, Dowbenko D, Jackson A, Toney L, Lewin DA, Dent AL, Lasky LA. The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. J Biol Chem. 2002 Apr 19;277(16):14255-65. Epub 2002 Jan 02.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-326

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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