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Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using Bcr (Phospho-Tyr177).
Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr (Phospho-Tyr177).
Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using Bcr (Phospho-Tyr177).

Bcr (phospho Tyr177) Antibody

CATALOG NUMBER: 79-194

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Bcr (Phospho-Tyr177) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y (p) -V-N) derived from Human Bcr.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.
Predicted Molecular Weight:
210 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BCR
Additional Names:
ALL, CML, PHL, BCR1, D22S11, D22S662, CFL, BCR, BCR protein
Protein Accession Number:
NP_004318.3
Protein Gi Number:
82546843
Ncbi Gene Id Number:
613
Background:
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Background Reference 1:
Sattler M, et al. Mol Cell Biol. 1999 Nov; 19 (11): 7473-7480. Li S et al. EMBO J. 2001 Dec 3; 20 (23): 6793-6804. Million RP, et, al. Mol Cell Biol. 2004 Jun; 24 (11): 4685-4695. Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 – 30916.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-194

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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