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To detect hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BD-2 (XP-5107Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-2.
To detect hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BD-2 (XP-5107Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-2.

BD-2 Antibody

CATALOG NUMBER: XP-5107

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2 (human BD-2).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBD-2.
Western Blot:
To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hBD-2 specific antibody was purified by affinity chromatography employing immobilized hBD-2 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
BD-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
DEFB4A
Additional Names:
BD-2, SAP1, DEFB2, DEFB4, HBD-2, DEFB-2, DEFB102, DEFB4B, Beta-defensin 4A, Beta-defensin 2, BD-2
Protein Accession Number:
O15263
Protein Gi Number:
3023383
Ncbi Gene Id Number:
1673
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5107

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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