Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in HepG2 cell line lysates (15ug/lane).

BDHA1 Antibody

CATALOG NUMBER: 61-972

Clonality:

Polyclonal

Tested Applications:

Flow, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rat

Immunogen:

This BDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human BDHA1.

Conjugate:

Unconjugated

Tested Applications:

Flow, WB

Application Note:

For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

43 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

physical-state:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

PDHA1

Additional Names:

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A

Protein Accession Number:

P08559

Protein Gi Number:

129063

Ncbi Gene Id Number:

5160

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The pyruvate dehydrogenase (PDH) complex is anuclear-encoded mitochondrial multienzyme complex that catalyzesthe overall conversion of pyruvate to acetyl-CoA and CO(2), andprovides the primary link between glycolysis and the tricarboxylicacid (TCA) cycle. The PDH complex is composed of multiple copies ofthree enzymatic components: pyruvate dehydrogenase (E1),dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3). The E1 enzyme is a heterotetramer of two alpha and two betasubunits. This gene encodes the E1 alpha 1 subunit containing theE1 active site, and plays a key role in the function of the PDHcomplex.

Background Reference 1:

Glushakova, L.G., et al. Mol. Genet. Metab. 98(3):289-299(2009)

Background Reference 2:

Joao Silva, M., et al. Eur. J. Pediatr. 168(1):17-22(2009)

Background Reference 3:

Boichard, A., et al. Mol. Genet. Metab. 93(3):323-330(2008)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-972

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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BDHA1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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