Western blot analysis of Actin in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, THP-1, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken small intestine, and drosophila lysate with Biotin-Beta-Actin antibody at 1 μg/mL.

beta-Actin Antibody (biotin)

CATALOG NUMBER: 3779-biotin

Clonality:

Polyclonal

Tested Applications:

ELISA, WB

Host Species:

Rabbit

Species Reactivity:

Chicken, Drosophila, Human, Mouse, Rabbit, Rat

Conjugate:

Biotin

Specifications

Host Species:

Rabbit

Species Reactivity:

Chicken, Drosophila, Human, Mouse, Rabbit, Rat

Immunogen:

Biotin-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

Conjugate:

Biotin

Tested Applications:

ELISA, WB

Application Note:

Biotin-Beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Specificity:

Biotin-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.

Positive Control 1:

Cat. No. 1201 - HeLa Cell Lysate

Predicted Molecular Weight:

Predicted: 42 kDa
Observed: 42 kDa

Advanced Validation

Properties

Purification:

Biotin-Beta-Actin antibody is affinity chromatography purified via peptide column.

Clonality:

Polyclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Biotin-Beta-Actin antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Concentration:

1 mg/mL

Storage Conditions:

Biotin-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Additional Info

Ncbi Official Symbol:

ACTB

Additional Names:

Biotin-Actin, Beta-Actin, Actin

Protein Accession Number:

AAH02409

Protein Gi Number:

12803203

Ncbi Gene Id Number:

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Background Reference 1:

Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.

Background Reference 2:

Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

View product citations for catalog number 3779-biotin on CiteAb

CATALOG NUMBER:

3779-biotin

List Size:

0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

List Size

Clear

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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beta-Actin Antibody (biotin)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Specificity:

Positive Control 1:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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