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Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL.
Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL.
Trial Size

beta-Galactosidase Antibody

CATALOG NUMBER: 5155

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Bacteria
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Bacteria
Immunogen:
beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal.
The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL.
Purification:
beta-Galactosidase Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
beta-Galactosidase antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
lacZ
Additional Names:
beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal
Protein Accession Number:
NP_752394
Protein Gi Number:
26246355
Ncbi Gene Id Number:
1036228
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
Background Reference 1:
Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5155

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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