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Western Blot at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

beta Tubulin

CATALOG NUMBER: 61-321

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Human, Pig
Immunogen:
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Tubb5
Additional Names:
Tubulin beta-5 chain, Tubb5
Protein Accession Number:
P99024
Ncbi Gene Id Number:
22154
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Background Reference 1:
Wang D.,et al.J. Cell Biol. 103:1903-1910(1986).
Background Reference 2:
Carninci P.,et al.Science 309:1559-1563(2005).
Background Reference 3:
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Background Reference 4:
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-321

List Size:
200 ul

List Price:

$495.00

Datasheet
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