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To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Betacellulin (38-180) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Betacellulin (38-180) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Betacellulin Antibody

CATALOG NUMBER: 38-179

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography employing immobilized hBetacellulin matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human Betacellulin (0.1 ng/mL), a concentration of ≤ 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect hBetacellulin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
Sandwich
To detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Betacellulin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Betacellulin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
BTC
Additional Names:
ProbetacellulinBTC
Protein Accession Number:
P35070
Protein Gi Number:
461653
Ncbi Gene Id Number:
685
User Note:
Centrifuge vial prior to opening.
Background:
Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-179

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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