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HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using 43-424 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDD
43-424 (2ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded
HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using 43-424 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDD

betaCstF-64 variant 1/ betaCstF-64 variant 3 Antibody

CATALOG NUMBER: 43-424

Clonality:
Polyclonal
Tested Applications:
ELISA, IP
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: C-PASIERVQGQRT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Immunoprecipitation: In transfected HeLa transiently expressing Mouse ßCstf-64 ((DYKDDDDK tagged), a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2ug per lysate.Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed ßCstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both ßCstf-64 anf Cstf-64 Recommended amount 2ug EB11838/500ug lysate protein.
Specificity:
This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
betaCstF-64 variant 1/ betaCstF-64 variant 3
Additional Names:
Cstf2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64kDa, C630034J23Rik, Cstf64, CF-1 64 kDa subunit, CSTF 64 kDa subunit, alphaCstF-64 variant 4, betaCstF-64 variant 1, betaCstF-64 variant 3, cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa, cstF-64
Protein Accession Number:
ACF05702.1,ACF05700.1
Protein Gi Number:
192764316
Ncbi Gene Id Number:
108062
Background Reference 1:
Shankarling GS, Coates PW, Dass B, Macdonald CC.A family of splice variants of CstF-64 expressed in vertebrate nervous systems.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-424

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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