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Western blot analysis of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35ug/lane)
BF Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35ug/lane)

BF Antibody

CATALOG NUMBER: 62-850

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human BF.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CFB
Additional Names:
Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, BF, BFD
Protein Accession Number:
P00751
Protein Gi Number:
584908
Ncbi Gene Id Number:
629
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BF is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
Background Reference 1:
Baatz,H., Klin Monatsbl Augenheilkd 226 (8), 654-658 (2009)
Background Reference 2:
Goring,K., Am. J. Physiol., Cell Physiol. 296 (5), C1140-C1150 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-850

List Size:
400 ul

List Price:

$495.00

Datasheet
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