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Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of BFAR in mouse kidney tissue with BFAR antibody at 5 μg/mL.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 μg/mL.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BFAR Antibody

CATALOG NUMBER: 5591

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BFAR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BFAR.
The immunogen is located within amino acids 300 - 350 of BFAR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
BFAR Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BFAR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BFAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BFAR
Additional Names:
BFAR Antibody: BAR, RNF47, BAR, Bifunctional apoptosis regulator, RING finger protein 47
Protein Accession Number:
NP_057645
Protein Gi Number:
7706091
Ncbi Gene Id Number:
51283
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BFAR Antibody: The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.
Background Reference 1:
Zhang H, Xu Q, Krajewski S, et al. BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins. Proc. Natl. Acad. Sci. USA2000; 97:2597-602.
Background Reference 2:
Roth W, Kermer P, Krajewska M, et al. Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways. Cell Death Differ. 2003; 10: 1178-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5591

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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