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Western blot analysis of Bfl-1 in (A) human kidney and (B) human lung tissue lysate with Bfl-1 antibody at 1 μg/mL.
Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 10 μg/mL.
Immunofluorescence of Bfl-1 in Mouse Kidney tissue with Bfl-1 antibody at 20 μg/mL.
Western blot analysis of Bfl-1 in (A) human kidney and (B) human lung tissue lysate with Bfl-1 antibody at 1 μg/mL.
Trial Size

Bfl-1 Antibody

CATALOG NUMBER: 3873

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1.
The immunogen is located within the last 50 amino acids of Bfl-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 3:
Cat. No. 1302 - Human Lung Tissue Lysate
Predicted Molecular Weight:
Predicted: 19 kDa
Observed: 20 kDa
Purification:
Bfl-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bfl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCL2A1
Additional Names:
Bfl-1 Antibody: GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5, GRS, Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5
Protein Accession Number:
NP_004040
Protein Gi Number:
4757840
Ncbi Gene Id Number:
597
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.
Background Reference 1:
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Background Reference 2:
Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.
Background Reference 3:
Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.
Background Reference 4:
Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3873

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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