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Western blot analysis of BGN using rabbit polyclonal BGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.
Western blot analysis of BGN Antibody in Sk-Br-3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with BGN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of BGN using rabbit polyclonal BGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.

BGN Antibody

CATALOG NUMBER: 62-440

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This BGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the Central region of human BGN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BGN
Additional Names:
Biglycan, Bone/cartilage proteoglycan I, PG-S1, BGN, SLRR1A
Protein Accession Number:
P21810
Protein Gi Number:
266762
Ncbi Gene Id Number:
633
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BGN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival.
Background Reference 1:
Sardo,M.A., Clin. Chim. Acta 406 (1-2), 89-93 (2009)
Background Reference 2:
Hwang,J.Y., Am. J. Pathol. 173 (6), 1919-1928 (2008)
Background Reference 3:
Traupe,H., Genomics 13 (2), 481-483 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-440

List Size:
400 ul

List Price:

$495.00

Datasheet
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