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Antibody used in WB on Rat liver at: 1:1000.
Antibody used in WB on Rat liver at: 1:2000.
Antibody used in WB on Human Fetal Liver at 2.5 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human 293T at 1 ug/ml.
Antibody used in WB on Rat liver at: 1:1000.

BHMT Antibody

CATALOG NUMBER: 29-583

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BHMT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Predicted Molecular Weight:
45 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store BHMT antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
BHMT
Additional Names:
BHMT, BHMT1, HEL-S-61p
Protein Accession Number:
NP_001704
Protein Gi Number:
157266337
Ncbi Gene Id Number:
635
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst (e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst (e)inemia,but such a defect has not yet been observed.
Background Reference 1:
Castro, C., (2004) Biochemistry 43 (18), 5341-5351.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-583

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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