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HEK293 overexpressing Human BICC1 with N-terminal FLAG, probed with rabbit anti-BCC1 in Western Blot after IP using either 43-143 or anti-FLAG antibody in the presence or absence of SDS.
HEK293 overexpressing Human BICC1 with N-terminal FLAG, probed with rabbit anti-BCC1 in Western Blot after IP using either 43-143 or anti-FLAG antibody in the presence or absence of SDS.

BICC1 Antibody

CATALOG NUMBER: 43-143

Clonality:
Polyclonal
Tested Applications:
ELISA, IP
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PNSPSIQHISQT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Kidney, Lung and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunoprecipitation:Immunoprecipitation by EB09818 from lysates of HEK293 transfected and overexpressing FLAG-HA-BICC1. Precipitation works best in the presence of SDS. Data kindly provided by anonymous customer.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BICC1
Additional Names:
BICC1, bicaudal C homolog 1 (Drosophila), DKFZp686I21119, bicaudal C homolog 1
Protein Accession Number:
NP_001073981.1
Protein Gi Number:
122937472
Ncbi Gene Id Number:
80114
Background Reference 1:
Riemenschneider M, Mahmoodzadeh S, Eisele T, Klopp N, Schwarz S, Wagenpfeil S, Diehl J, Mueller U, Foerstl H, Illig T, Kurz A, Association analysis of genes involved in cholesterol metabolism located within the linkage region on chromosome 10 and Alzheimer's disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-143

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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