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Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 μg/mL.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 μg/mL.
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

Bid Antibody

CATALOG NUMBER: 3353

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Bid antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Bid.
The immunogen is located within amino acids 120 - 170 of Bid.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Purification:
Bid Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bid Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bid antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BID
Additional Names:
Bid Antibody: FP497, BH3-interacting domain death agonist, p22 BID, BID
Protein Accession Number:
AAH36364
Protein Gi Number:
54673639
Ncbi Gene Id Number:
637
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bid Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals, exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein. The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize.
Background Reference 1:
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Background Reference 2:
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Background Reference 3:
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Background Reference 4:
Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3353

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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