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45-143 staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-143 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-143 staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

BIF-1 Antibody

CATALOG NUMBER: 45-143

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QKGKVPITYLELLN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SH3GLB1
Additional Names:
SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2
Protein Accession Number:
NP_057093.1
Protein Gi Number:
7705773
Ncbi Gene Id Number:
51100
Background Reference 1:
Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol Cell Biol. 2005 Nov;25(21):9369-82.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-143

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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