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45-329 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
45-329 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Biglycan Preproprotein Antibody

CATALOG NUMBER: 45-329

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DRLAIQFGNYKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Expression of the protein seen in the Golgi of NIH3T3 and HepG2 cells. Recommended concentration: 10µg/ml.
Positive Control 1:
Cat. No. 1480 - Rat Skin Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BGN
Additional Names:
Biglycan Preproprotein, biglycan, BGN, DSPG1, PG-S1, PGI, SLRR1A , bone/cartilage proteoglycan-I, dermatan sulphate proteoglycan I, small leucine-rich protein 1A
Protein Accession Number:
NP_001702.1
Protein Gi Number:
4502403
Ncbi Gene Id Number:
633
Background Reference 1:
Chen WB, Lenschow W, Tiede K, Fischer JW, Kalthoff H, Ungefroren H. Smad4/DPC4-dependent regulation of biglycan gene expression by transforming growth factor-beta in pancreatic tumor cells. J Biol Chem. 2002 Sep 27;277(39):36118-28. Epub 2002 Jul 24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-329

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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