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Antibody is used in Western blot to detect Bike in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect Bike in mouse liver tissue lysate.

BIKE Antibody

CATALOG NUMBER: 59-055

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This BIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-291 amino acids from the Central region of human BIKE.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
129 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BMP2K
Additional Names:
BMP-2-inducible protein kinase, BIKe, BMP2K, BIKE
Protein Accession Number:
Q9NSY1
Protein Gi Number:
34222653
Ncbi Gene Id Number:
55589
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BIKE is the human homolog of mouse BMP-2-inducible kinase. Bone morphogenic proteins (BMPs) play a key role in skeletal development and patterning. Expression of the mouse gene is increased during BMP-2 induced differentiation and the gene product is a putative serine/threonine protein kinase containing a nuclear localization signal. Therefore, the protein encoded by this human homolog is thought to be a protein kinase with a putative regulatory role in attenuating the program of osteoblast differentiation.
Background Reference 1:
Kearns, A.E., et al., J. Biol. Chem. 276(45):42213-42218 (2001).
Background Reference 2:
Hoffmann, A., et al., Crit. Rev. Eukaryot. Gene Expr. 11 (1-3), 23-45 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-055

List Size:
400 ul

List Price:

$495.00

Datasheet
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