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45-330 staining (0.5ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-330 staining (0.5ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

BIM Antibody

CATALOG NUMBER: 45-330

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-FNAYYARRLEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 23kDa band observed in K562 lysates (predicted MW of 18.5kDa according to NP_619528.1). Recommended for use at 0.5-2ug/ml.
Specificity:
This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BCL2L11
Additional Names:
BCL2L11, BCL2-like 11 (apoptosis facilitator), BAM, BIM, BOD, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, bcl-2-like protein 11, bcl-2-related ovarian death agonist, bcl2-L-11, Bim-alpha1, Bim-alpha2, Bim-alpha3
Protein Accession Number:
NP_619528.1,NP_619529.1,NP_996886.1,
Protein Gi Number:
20336317
Ncbi Gene Id Number:
10018
Background Reference 1:
Marani M, Tenev T, Hancock D, Downward J, Lemoine NR. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol. 2002 Jun;22(11):3577-89.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-330

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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