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Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).
Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).

Biotin Antibody [SPM375]

CATALOG NUMBER: 34-197

Clonality:
Monoclonal
Tested Applications:
CM, Flow, Functional Assays, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
All Species
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
All Species
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Conjugate:
Unconjugated
Tested Applications:
CM, Flow, Functional Assays, IF, IHC, WB
Application Note:
Confocal Microscopy
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
Western: blot 1-3 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-197

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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