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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis of BIRC3 using rabbit polyclonal BIRC3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.
Confocal immunofluorescent analysis of BIRC3 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
BIRC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

BIRC3 Antibody

CATALOG NUMBER: 57-421

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BIRC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human BIRC3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BIRC3
Additional Names:
Baculoviral IAP repeat-containing protein 3, 632-, Apoptosis inhibitor 2, API2, C-IAP2, IAP homolog C, Inhibitor of apoptosis protein 1, IAP-1, hIAP-1, hIAP1, RING finger protein 49, TNFR2-TRAF-signaling complex protein 1, BIRC3, API2, IAP1, MIHC, RNF49
Protein Accession Number:
Q13489
Protein Gi Number:
2497236
Ncbi Gene Id Number:
330
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of a familyof proteins that inhibits apoptosis by binding to tumor necrosisfactor receptor-associated factors TRAF1 and TRAF2, probably byinterfering with activation of ICE-like proteases. The encodedprotein inhibits apoptosis induced by serum deprivation but doesnot affect apoptosis resulting from exposure to menadione, a potentinducer of free radicals. The amino acid sequence predicts threebaculovirus IAP repeat domains and a ring finger domain. Transcriptvariants encoding the same isoform have been identified. [providedby RefSeq].
Background Reference 1:
Zane, L., et al. Virology 407(2):341-351(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Kim, C.W., et al. Biochem. Biophys. Res. Commun. 400(1):46-52(2010)
Background Reference 4:
Petersen, S.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11936-11941(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-421

List Size:
400 ul

List Price:

$495.00

Datasheet
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